文章摘要：培养基成分及其用途1.AcetobacterMedium(醋酸菌培养基)Glucose(葡萄糖)100gYetextract(酵母膏)10gCaCO320gAgar(琼脂)15gDistilledwater(蒸馏水)1000mlAdjust(调)pHto6.8适用范围：恶臭醋酸杆......
bsp;183
脱脂牛奶 100g 去离子水 1000ml
适用范围：保加利亚乳杆菌、嗜热乳酸链球菌
45. Pea Agar (豌豆琼脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (琼脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2计)] 12-14 mg∕100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (称取白豌豆900克，置于5公升广口瓶中，加自来水3公升，用硫酸调pH至4.3-4.5，再加氯仿30毫升（防腐），加胶塞并用纱布扎口（防止冲脱），放37℃恒温室7-10天。取出用滤纸过滤，滤液煮沸20分钟，以除氯仿，并再次以滤纸过滤，除沉淀。滤液分装，于120℃30分钟灭菌。后冷藏备用。)
The standard quality of pea infusion as following (豌豆浸汁质量标准)：
Aminonitrogen（氨基酸）: 160-200 mg∕100ml
Soluble phosphoruse（溶磷）: 400-600 μg∕ml
Color（色泽）: auratus,transparent （金黄、透明）
46. Wheat Bran Medium (麦麸琼脂)
Wheat bran (麦麸) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (琼脂) 2% pH 7.0
适用范围：金霉素链霉菌、红霉素链霉菌
47. Glucose Yeast Extract Agar (葡萄糖、酵母膏琼脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Water (水) 1000ml Agar (琼脂) 15g
pH 7.2
48. MMN Medium (MMN培养基)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg
(NH4)2HPO4 0.25g Wort (12 Be′) 100ml
Glucose (葡萄糖) 10-15g Citric acid (柠檬酸) 0.2g
Distilled water (蒸馏水) 900ml pH 5.5 (左右)
适用范围：蜜环菌
49. Starch Agar (淀粉琼脂)
Soluble starch (可溶性淀粉) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (琼脂) 20g
Distilled water (蒸馏水) 1000ml
50. 5 Be′Wort A上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] ... 下一页 >>
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